› 4z4s

X-ray diffraction
1.8Å resolution

Crystal structure of GII.10 P domain in complex with 150mM fucose

Released:
DOI: 10.2210/pdb4z4s/pdb
PDB entry 4z4s coloured by chain, front view.
PDB entry 4z4s coloured by chain, side view.
PDB entry 4z4s coloured by chain, top view.
Source organism: Norovirus GII.10
Primary publication:
The sweet quartet: Binding of fucose to the norovirus capsid.
Koromyslova AD, Leuthold MM, Bowler MW, Hansman GS
Virology 483 203-8 (2015)
PMID: 25980740

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norovirus GII.10
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 7 x EDO
Ligand FUL 4 x FUL
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 66.09Å b: 78.681Å c: 68.811Å
α: 90° β: 99.61° γ: 90°
R-values:
R R work R free
0.147 0.145 0.182
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Human Norovirus Interactions with Histo-Blood Group Antigens and Human Milk Oligosaccharides.
Schroten et al. (2016)
4 more

1 mention without citation

Glycan-Induced Protein Dynamics in Human Norovirus P Dimers Depend on Virus Strain and Deamidation Status.
Dülfer et al. (2021)