› 4z3o

X-ray diffraction
3.44Å resolution

Quinolone(Moxifloxacin)-DNA cleavage complex of topoisomerase IV from S. pneumoniae

Released:
DOI: 10.2210/pdb4z3o/pdb
PDB entry 4z3o coloured by chain, front view.
PDB entry 4z3o coloured by chain, side view.
PDB entry 4z3o coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Laponogov I, Veselkov DA, Pan X-S, Selvarajah J, Crevel IM-T, Fisher LM, Sanderson MR

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118351 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
DNA topoisomerase 4 subunit A; DNA topoisomerase 4 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 742 amino acids
Theoretical weight: 84.21 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59961 (Residues: 404-643; Coverage: 37%)
  • Canonical: P72525 (Residues: 3-484; Coverage: 59%)
Gene names: SP_0852, SP_0855, parC, parE
Sequence domains:
Structure domains:
E-site DNA Chain: E
Molecule details ›
Chain: E
Length: 7 nucleotides
Theoretical weight: 2.12 KDa
E-site DNA Chain: F
Molecule details ›
Chain: F
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
E-site DNA Chain: G
Molecule details ›
Chain: G
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
E-site DNA Chain: H
Molecule details ›
Chain: H
Length: 11 nucleotides
Theoretical weight: 3.36 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand MFX 2 x MFX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3121
Unit cell:
a: 157.76Å b: 157.76Å c: 211.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Escherichia coli

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