› 4z28

X-ray diffraction
1.66Å resolution

Crystal structure of short hoefavidin biotin complex

Released:
DOI: 10.2210/pdb4z28/pdb
PDB entry 4z28 coloured by chain, front view.
PDB entry 4z28 coloured by chain, side view.
PDB entry 4z28 coloured by chain, top view.
Source organism: Hoeflea phototrophica DFL-43
Primary publication:
Hoefavidin: A dimeric bacterial avidin with a C-terminal binding tail.
Avraham O, Meir A, Fish A, Bayer EA, Livnah O
J Struct Biol 191 139-48 (2015)
PMID: 26126731

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hoefavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 14.34 KDa
Source organism: Hoeflea phototrophica DFL-43
Expression system: Escherichia coli
UniProt:
  • Canonical: A9D857 (Residues: 21-154; Coverage: 93%)
Gene name: HPDFL43_17171
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BTN 2 x BTN
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 61.18Å b: 61.18Å c: 166.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.23
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis.
Jain et al. (2017)
8 other articles

1 mention without citation

Structural characterization of core-bradavidin in complex with biotin.
Agrawal et al. (2017)