› 4yzg

X-ray diffraction
1.6Å resolution

Structure of the Arabidopsis TAP38/PPH1, a state-transition phosphatase responsible for dephosphorylation of LHCII

Released:
DOI: 10.2210/pdb4yzg/pdb
PDB entry 4yzg coloured by chain, front view.
PDB entry 4yzg coloured by chain, side view.
PDB entry 4yzg coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1.
Wei X, Guo J, Li M, Liu Z
Plant Cell 27 1113-27 (2015)
PMID: 25888588

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein phosphatase 2C 57 Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.71 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49599 (Residues: 59-351; Coverage: 76%)
Gene names: At4g27800, PP2C57, PPH1, T27E11.40, TAP38
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 55.399Å b: 73.879Å c: 81.998Å
α: 90° β: 109.15° γ: 90°
R-values:
R R work R free
0.181 0.18 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Composition, phosphorylation and dynamic organization of photosynthetic protein complexes in plant thylakoid membrane.
Rantala et al. (2020)
3 more

1 mention without citation

Dynamic Regulation of the Light-Harvesting System through State Transitions in Land Plants and Green Algae.
Shang et al. (2023)