› 4yw6

X-ray diffraction
1.4Å resolution

Structural Insight into Divalent Galactoside Binding to Pseudomonas aeruginosa lectin LecA

Released:
DOI: 10.2210/pdb4yw6/pdb
PDB entry 4yw6 coloured by chain, front view.
PDB entry 4yw6 coloured by chain, side view.
PDB entry 4yw6 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural Insight into Multivalent Galactoside Binding to Pseudomonas aeruginosa Lectin LecA.
Visini R, Jin X, Bergmann M, Michaud G, Pertici F, Fu O, Pukin A, Branson TR, Thies-Weesie DM, Kemmink J, Gillon E, Imberty A, Stocker A, Darbre T, Pieters RJ, Reymond JL
ACS Chem Biol 10 2455-62 (2015)
PMID: 26295304

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA-I galactophilic lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand G0P 4 x G0P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 40.87Å b: 72.98Å c: 79.02Å
α: 117.52° β: 104.99° γ: 90°
R-values:
R R work R free
0.186 0.186 0.206
Expression system: Escherichia coli

Quick links

Citations

7 review citations

From Nanobiotechnology, Positively Charged Biomimetic Dendrimers as Novel Antibacterial Agents: A Review.
Alfei et al. (2020)
6 more