› 4yru

X-ray diffraction
2.8Å resolution

Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus

Released:
DOI: 10.2210/pdb4yru/pdb
PDB entry 4yru coloured by chain, front view.
PDB entry 4yru coloured by chain, side view.
PDB entry 4yru coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
Pandalaneni S, Karuppiah V, Saleem M, Haynes LP, Burgoyne RD, Mayans O, Derrick JP, Lian LY
J Biol Chem 290 18744-56 (2015)
PMID: 25979333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal calcium sensor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 20.54 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62168 (Residues: 1-177; Coverage: 93%)
Gene names: Flup, Freq, Ncs1
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 72.68Å b: 88.8Å c: 100.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.247 0.291
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne et al. (2019)
4 more

3 mentions without citation

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
Pandalaneni et al. (2015)
2 more