› 4yma

X-ray diffraction
1.9Å resolution

Structure of the ligand-binding domain of GluA2 in complex with the antagonist CNG10109

Released:
DOI: 10.2210/pdb4yma/pdb
PDB entry 4yma coloured by chain, front view.
PDB entry 4yma coloured by chain, side view.
PDB entry 4yma coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure-Activity Relationship Study of Ionotropic Glutamate Receptor Antagonist (2S,3R)-3-(3-Carboxyphenyl)pyrrolidine-2-carboxylic Acid.
Krogsgaard-Larsen N, Storgaard M, Møller C, Demmer CS, Hansen J, Han L, Monrad RN, Nielsen B, Tapken D, Pickering DS, Kastrup JS, Frydenvang K, Bunch L
J Med Chem 58 6131-50 (2015)
PMID: 26200741

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.28 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-797; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 2 x GOL
Ligand 4E5 2 x 4E5
Ligand EDO 2 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 48.88Å b: 65.25Å c: 91.55Å
α: 90° β: 92.36° γ: 90°
R-values:
R R work R free
0.173 0.17 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
1 more

1 mention without citation

Stereoselective Synthesis of New (2S,3R)-3-Carboxyphenyl)pyrrolidine-2-carboxylic Acid Analogues Utilizing a C(sp3)-H Activation Strategy and Structure-Activity Relationship Studies at the Ionotropic Glutamate Receptors.
Kayser et al. (2020)