› 4yb6

X-ray diffraction
1.98Å resolution

Adenosine triphosphate phosphoribosyltransferase from Campylobacter jejuni in complex with the inhibitors AMP and histidine

Released:
DOI: 10.2210/pdb4yb6/pdb
PDB entry 4yb6 coloured by chain, front view.
PDB entry 4yb6 coloured by chain, side view.
PDB entry 4yb6 coloured by chain, top view.
Source organism: Campylobacter jejuni RM1221
Primary publication:
Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer that is allosterically controlled by the twisting of a regulatory tail.
Mittelstädt G, Moggré GJ, Panjikar S, Nazmi AR, Parker EJ
Protein Sci 25 1492-506 (2016)
PMID: 27191057

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP phosphoribosyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 300 amino acids
Theoretical weight: 33.74 KDa
Source organism: Campylobacter jejuni RM1221
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5HSJ4 (Residues: 1-299; Coverage: 100%)
Gene names: CJE1769, hisG
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand AMP 6 x AMP
Ligand HIS 6 x HIS
Ligand PEG 6 x PEG
Ligand MG 9 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 91.871Å b: 124.906Å c: 92.809Å
α: 90° β: 115.86° γ: 90°
R-values:
R R work R free
0.196 0.195 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase.
Mittelstädt et al. (2018)
6 more

4 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
3 more