› 4y7j

X-ray diffraction
4.1Å resolution

Structure of an archaeal mechanosensitive channel in expanded state

Released:
DOI: 10.2210/pdb4y7j/pdb
PDB entry 4y7j coloured by chain, front view.
PDB entry 4y7j coloured by chain, side view.
PDB entry 4y7j coloured by chain, top view.
Source organisms:
Primary publication:
Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion.
Li J, Guo J, Ou X, Zhang M, Li Y, Liu Z
Proc Natl Acad Sci U S A 112 10726-31 (2015)
PMID: 26261325

Function and Biology Details

Reaction catalysed: 
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large conductance mechanosensitive channel protein; Riboflavin synthase Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 277 amino acids
Theoretical weight: 30.87 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TNK0 (Residues: 1-101; Coverage: 100%)
  • Canonical: Q58584 (Residues: 1-156; Coverage: 100%)
Gene names: MA_2285, MJ1184, ribC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand BNG 1 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21212
Unit cell:
a: 147.36Å b: 149.25Å c: 99.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.323 0.321 0.376
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Discoveries in structure and physiology of mechanically activated ion channels.
Kefauver et al. (2020)
3 more

7 mentions without citation

Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.
Blount et al. (2020)
6 more