› 4xzw

X-ray diffraction
1.5Å resolution

Endo-glucanase chimera C10

Released:
DOI: 10.2210/pdb4xzw/pdb
PDB entry 4xzw coloured by chain, front view.
PDB entry 4xzw coloured by chain, side view.
PDB entry 4xzw coloured by chain, top view.
Source organisms:
Entry authors: Lee CC, Chang CJ, Ho THD, Chao YC, Wang AHJ

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase; cellulase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.45 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M4I2R5 (Residues: 32-42, 52-55, 117-123, 158-174, 228-250, 253-265, 269-291; Coverage: 26%)
  • Canonical: C5H6X3 (Residues: 37-45, 50-113, 121-154, 173-225, 249-250, 264-266, 290-330; Coverage: 53%)
Gene name: celA
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand O4B 2 x O4B
Ligand CA 1 x CA
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: C2221
Unit cell:
a: 63.251Å b: 83.248Å c: 121.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.2
Expression system: Escherichia coli BL21(DE3)

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