› 4xyg

X-ray diffraction
1.7Å resolution

GRANULICELLA M. FORMATE DEHYDROGENASE (FDH)

Released:
DOI: 10.2210/pdb4xyg/pdb
PDB entry 4xyg coloured by chain, front view.
PDB entry 4xyg coloured by chain, side view.
PDB entry 4xyg coloured by chain, top view.
Source organism: Granulicella mallensis MP5ACTX8
Primary publication:
Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8.
Fogal S, Beneventi E, Cendron L, Bergantino E
Appl Microbiol Biotechnol 99 9541-54 (2015)
PMID: 26104866

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 42.81 KDa
Source organism: Granulicella mallensis MP5ACTX8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G8NVB5 (Residues: 1-391; Coverage: 100%)
Gene name: AciX8_2172
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3121
Unit cell:
a: 60.166Å b: 60.166Å c: 179.683Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP.
Calzadiaz-Ramirez et al. (2020)
7 more