› 4xt5

X-ray diffraction
2.13Å resolution

Crystal structure of Rv2671 from Mycobacterium tuberculosis in complex with NADP+

Released:
DOI: 10.2210/pdb4xt5/pdb
PDB entry 4xt5 coloured by chain, front view.
PDB entry 4xt5 coloured by chain, side view.
PDB entry 4xt5 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural Insights into Mycobacterium tuberculosis Rv2671 Protein as a Dihydrofolate Reductase Functional Analogue Contributing to para-Aminosalicylic Acid Resistance.
Cheng YS, Sacchettini JC
Biochemistry 55 1107-19 (2016)
PMID: 26848874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial bifunctional deaminase-reductase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.72 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P71968 (Residues: 1-258; Coverage: 100%)
Gene names: Rv2671, ribD, ribG
Sequence domains: RibD C-terminal domain
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 69.697Å b: 95.26Å c: 73.854Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New prodrugs against tuberculosis.
Mori et al. (2017)
1 more

1 mention without citation

Structural Insights into Mycobacterium tuberculosis Rv2671 Protein as a Dihydrofolate Reductase Functional Analogue Contributing to para-Aminosalicylic Acid Resistance.
Cheng et al. (2016)