› 4xoj

X-ray diffraction
0.91Å resolution

Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)

Released:
DOI: 10.2210/pdb4xoj/pdb
PDB entry 4xoj coloured by chain, front view.
PDB entry 4xoj coloured by chain, side view.
PDB entry 4xoj coloured by chain, top view.
Source organisms:
Primary publication:
Investigation of Serine-Proteinase-Catalyzed Peptide Splicing in Analogues of Sunflower Trypsin Inhibitor 1 (SFTI-1).
Karna N, Łęgowska A, Malicki S, Dębowski D, Golik P, Gitlin A, Grudnik P, Wladyka B, Brzozowski K, Dubin G, Rolka K
Chembiochem 16 2036-45 (2015)
PMID: 26212347

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133355 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 25.81 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 1-246; Coverage: 100%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Trypsin inhibitor 1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.42 KDa
Source organism: Helianthus annuus
Expression system: Not provided
UniProt:
  • Canonical: Q4GWU5 (Residues: 40-52; Coverage: 42%)
Gene name: sfti1

Ligands and Environments

5 bound ligands:
Ligand SO4 1 x SO4
Ligand EDO 7 x EDO
Ligand CA 1 x CA
Ligand NA 3 x NA
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 62.39Å b: 63.05Å c: 69.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.104 0.103 0.114
Expression system: Not provided

Quick links

Citations

2 citation in other articles

Spliced analogues of trypsin inhibitor SFTI-1 and their application for tracing proteolysis and delivery of cargos inside the cells.
Filipowicz et al. (2017)
1 more

3 mentions without citation

Structural Determinants of Substrate Specificity of SplF Protease from Staphylococcus aureus.
Stach et al. (2021)
2 more