› 4xnx

X-ray diffraction
3Å resolution

X-ray structure of Drosophila dopamine transporter in complex with reboxetine

Released:
DOI: 10.2210/pdb4xnx/pdb
PDB entry 4xnx coloured by chain, front view.
PDB entry 4xnx coloured by chain, side view.
PDB entry 4xnx coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of Drosophila dopamine transporter in complex with nisoxetine and reboxetine.
Penmatsa A, Wang KH, Gouaux E
Nat Struct Mol Biol 22 506-508 (2015)
PMID: 25961798

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230374 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 60.15 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
antibody fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Antibody fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.92 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
4 bound ligands:
Ligand CLR 2 x CLR
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand 41X 1 x 41X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 97.673Å b: 141.036Å c: 167.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.258
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

24 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
23 more

11 mentions without citation

Structure and Gating Dynamics of Na+/Cl- Coupled Neurotransmitter Transporters.
Joseph et al. (2019)
10 more