› 4xl4

X-ray diffraction
1.9Å resolution

Crystal structure of thiolase from Clostridium acetobutylicum in complex with CoA

Released:
DOI: 10.2210/pdb4xl4/pdb
PDB entry 4xl4 coloured by chain, front view.
PDB entry 4xl4 coloured by chain, side view.
PDB entry 4xl4 coloured by chain, top view.
Source organism: Clostridium acetobutylicum EA 2018
Primary publication:
Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum.
Kim S, Jang YS, Ha SC, Ahn JW, Kim EJ, Lim JH, Cho C, Ryu YS, Lee SK, Lee SY, Kim KJ
Nat Commun 6 8410 (2015)
PMID: 26391388

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 42.35 KDa
Source organism: Clostridium acetobutylicum EA 2018
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0R4I970 (Residues: 1-392; Coverage: 98%)
Gene name: CEA_G2880
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21212
Unit cell:
a: 204.307Å b: 54.171Å c: 73.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.183
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Allosteric disulfides: Sophisticated molecular structures enabling flexible protein regulation.
Chiu et al. (2019)
7 more

2 mentions without citation

Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum.
Kim et al. (2015)
1 more