› 4xkv

X-ray diffraction
2.1Å resolution

Tailspike protein mutant D339N of E. coli bacteriophage HK620

Released:
DOI: 10.2210/pdb4xkv/pdb
PDB entry 4xkv coloured by chain, front view.
PDB entry 4xkv coloured by chain, side view.
PDB entry 4xkv coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Entry authors: Gohlke U, Broeker NK, Heinemann U, Seckler R, Barbirz S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 64.25 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AYY6 (Residues: 115-710; Coverage: 84%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TRS 1 x TRS
Ligand FMT 3 x FMT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 73.917Å b: 73.917Å c: 174.502Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.227 0.282
Expression system: Escherichia coli BL21

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