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X-ray diffraction
3Å resolution

Kinase and Dimerization (P3P4) of the Thermotoga maritima CheA kinase

Released:
DOI: 10.2210/pdb4xiv/pdb
PDB entry 4xiv coloured by chain, front view.
PDB entry 4xiv coloured by chain, side view.
PDB entry 4xiv coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Conformational Transitions that Enable Histidine Kinase Autophosphorylation and Receptor Array Integration.
Greenswag AR, Muok A, Li X, Crane BR
J Mol Biol 427 3890-907 (2015)
PMID: 26522934

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheA Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.5 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56310 (Residues: 289-540; Coverage: 38%)
Gene names: TM_0702, cheA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 66.412Å b: 131.405Å c: 147.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.282
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
3 more

5 mentions without citation

Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations.
Cassidy et al. (2020)
4 more