› 4xih

X-ray diffraction
2.25Å resolution

Crystal structure of the R116A mutant AhpE from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4xih/pdb
PDB entry 4xih coloured by chain, front view.
PDB entry 4xih coloured by chain, side view.
PDB entry 4xih coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CCDC5079
Primary publication:
The active site architecture in peroxiredoxins: a case study on Mycobacterium tuberculosis AhpE.
Pedre B, van Bergen LA, Palló A, Rosado LA, Dufe VT, Molle IV, Wahni K, Erdogan H, Alonso M, Proft FD, Messens J
Chem Commun (Camb) 52 10293-6 (2016)
PMID: 27471753

Function and Biology Details

Reaction catalysed: 
Mycoredoxin + ROOH = mycoredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.81 KDa
Source organism: Mycobacterium tuberculosis CCDC5079
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE3 (Residues: 1-152; Coverage: 99%)
Gene names: MTCY427.19c, Rv2238c, ahpE
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I4
Unit cell:
a: 147.26Å b: 147.26Å c: 33.303Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.224
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Computational Tools to Rationalize and Predict the Self-Assembly Behavior of Supramolecular Gels.
Van Lommel et al. (2021)
5 other articles

1 mention without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)