› 4xgz

X-ray diffraction
2.5Å resolution

Crystal structure of human paxillin LD2 motif in complex with Fab fragment

Released:
DOI: 10.2210/pdb4xgz/pdb
PDB entry 4xgz coloured by chain, front view.
PDB entry 4xgz coloured by chain, side view.
PDB entry 4xgz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.
Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA
J Mol Biol 427 2532-2547 (2015)
PMID: 26087144

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229963 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB HEAVY CHAIN Chains: A, C, E, G, H, J, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, H, J, M, O, Q, S, U, W
Length: 229 amino acids
Theoretical weight: 24.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB LIGHT CHAIN Chains: B, D, F, I, K, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, I, K, L, N, P, R, T, V, X
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Paxillin Chains: a, c, e, g, h, j, m, o, q, s, u, w
Molecule details ›
Chains: a, c, e, g, h, j, m, o, q, s, u, w
Length: 19 amino acids
Theoretical weight: 2.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P49023 (Residues: 141-159; Coverage: 3%)
Gene name: PXN

Ligands and Environments

2 bound ligands:
Ligand NO3 12 x NO3
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 108.189Å b: 111.004Å c: 143.865Å
α: 94.65° β: 95.28° γ: 114.53°
R-values:
R R work R free
0.228 0.228 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.
Derewenda et al. (2017)
2 more

3 mentions without citation

Mechanism of integrin activation by talin and its cooperation with kindlin.
Lu et al. (2022)
2 more