› 4x89

X-ray diffraction
2.62Å resolution

NavMs voltage-gated sodium channal pore and C-terminal domain soaked with Silver nitrate

Released:
DOI: 10.2210/pdb4x89/pdb
PDB entry 4x89 coloured by chain, front view.
PDB entry 4x89 coloured by chain, side view.
PDB entry 4x89 coloured by chain, top view.
Source organism: Magnetococcus marinus MC-1
Primary publication:
Molecular basis of ion permeability in a voltage-gated sodium channel.
Naylor CE, Bagnéris C, DeCaen PG, Sula A, Scaglione A, Clapham DE, Wallace BA
EMBO J 35 820-30 (2016)
PMID: 26873592

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 149 amino acids
Theoretical weight: 16.84 KDa
Source organism: Magnetococcus marinus MC-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0L5S6 (Residues: 130-274; Coverage: 53%)
Gene name: Mmc1_0798
Sequence domains: Ion transport protein
Structure domains: Helix Hairpins

Ligands and Environments

4 bound ligands:
Ligand 2CV 4 x 2CV
Ligand 1PE 4 x 1PE
Ligand NA 7 x NA
Ligand AG 1 x AG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 80Å b: 329.24Å c: 80.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

The chemical basis for electrical signaling.
Catterall et al. (2017)
14 more