› 4x7w

X-ray diffraction
1.75Å resolution

MycF mycinamicin III 3'-O-methyltransferase (E35Q, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)

Released:
DOI: 10.2210/pdb4x7w/pdb
PDB entry 4x7w coloured by chain, front view.
PDB entry 4x7w coloured by chain, side view.
PDB entry 4x7w coloured by chain, top view.
Source organism: Micromonospora griseorubida
Primary publication:
Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases.
Bernard SM, Akey DL, Tripathi A, Park SR, Konwerski JR, Anzai Y, Li S, Kato F, Sherman DH, Smith JL
ACS Chem Biol 10 1340-51 (2015)
PMID: 25692963

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + mycinamicin III = S-adenosyl-L-homocysteine + mycinamicin IV
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycinamicin III 3''-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.76 KDa
Source organism: Micromonospora griseorubida
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q49492 (Residues: 1-254; Coverage: 100%)
Gene name: mycF
Sequence domains: Macrocin-O-methyltransferase (TylF)

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
2 bound ligands:
Ligand MG 2 x MG
Ligand MVI 2 x MVI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 50.121Å b: 92.528Å c: 128.926Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.209
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Methyltransferases: Functions and Applications.
Abdelraheem et al. (2022)
6 other articles

1 mention without citation

Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases.
Bernard et al. (2015)