› 4x7b

X-ray diffraction
2.4Å resolution

Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant H86Y/A269T

Released:
DOI: 10.2210/pdb4x7b/pdb
PDB entry 4x7b coloured by chain, front view.
PDB entry 4x7b coloured by chain, side view.
PDB entry 4x7b coloured by chain, top view.
Source organism: Geobacillus stearothermophilus T6
Primary publication:
Structural insights into methanol-stable variants of lipase T6 from Geobacillus stearothermophilus.
Dror A, Kanteev M, Kagan I, Gihaz S, Shahar A, Fishman A
Appl Microbiol Biotechnol 99 9449-61 (2015)
PMID: 26026940

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 43.9 KDa
Source organism: Geobacillus stearothermophilus T6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93A71 (Residues: 34-418; Coverage: 92%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 49.81Å b: 71.75Å c: 114.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Bacterial lipases: A review on purification and characterization.
Javed et al. (2018)
2 more