› 4wyu

X-ray diffraction
2.5Å resolution

Crystal Structure of Scribble PDZ34 tandem in complex with its target peptide

Released:
DOI: 10.2210/pdb4wyu/pdb
PDB entry 4wyu coloured by chain, front view.
PDB entry 4wyu coloured by chain, side view.
PDB entry 4wyu coloured by chain, top view.
Source organisms:
Primary publication:
Interdomain interface-mediated target recognition by the Scribble PDZ34 supramodule.
Ren J, Feng L, Bai Y, Pei H, Yuan Z, Feng W
Biochem J 468 133-44 (2015)
PMID: 25734361

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein scribble homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14160 (Residues: 992-1203; Coverage: 13%)
Gene names: CRIB1, KIAA0147, LAP4, SCRB1, SCRIB, VARTUL
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
peptide SER-TRP-PHE-GLN-THR-ASP-LEU Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 896 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IOD 7 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 37.27Å b: 78.43Å c: 94.08Å
α: 90° β: 99.51° γ: 90°
R-values:
R R work R free
0.191 0.189 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Viral subversion of the cell polarity regulator Scribble.
Javorsky et al. (2023)
6 other articles

2 mentions without citation

PDZ Domains as Drug Targets.
Christensen et al. (2019)
1 more