› 4whm

X-ray diffraction
1.85Å resolution

Crystal structure of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in complex with UDP

Released:
DOI: 10.2210/pdb4whm/pdb
PDB entry 4whm coloured by chain, front view.
PDB entry 4whm coloured by chain, side view.
PDB entry 4whm coloured by chain, top view.
Source organism: Clitoria ternatea
Primary publication:
Structural basis for acceptor-substrate recognition of UDP-glucose: anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea.
Hiromoto T, Honjo E, Noda N, Tamada T, Kazuma K, Suzuki M, Blaber M, Kuroki R
Protein Sci 24 395-407 (2015)
PMID: 25556637

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 48.78 KDa
Source organism: Clitoria ternatea
Expression system: Escherichia coli
UniProt:
  • Canonical: A4F1R4 (Residues: 1-446; Coverage: 100%)
Gene name: Ct3GT-A
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand UDP 1 x UDP
Ligand GOL 2 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 50.059Å b: 55.335Å c: 85.517Å
α: 90° β: 105.04° γ: 90°
R-values:
R R work R free
0.153 0.151 0.187
Expression system: Escherichia coli

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Citations

4 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
3 more

2 mentions without citation

Structure-function and engineering of plant UDP-glycosyltransferase.
Wang et al. (2023)
1 more