› 4wh4

X-ray diffraction
2.2Å resolution

Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H

Released:
DOI: 10.2210/pdb4wh4/pdb
PDB entry 4wh4 coloured by chain, front view.
PDB entry 4wh4 coloured by chain, side view.
PDB entry 4wh4 coloured by chain, top view.
Source organism: Streptococcus sp. 'group G'
Primary publication:
The double-histidine Cu²⁺-binding motif: a highly rigid, site-specific spin probe for electron spin resonance distance measurements.
Cunningham TF, Putterman MR, Desai A, Horne WS, Saxena S
Angew Chem Int Ed Engl 54 6330-4 (2015)
PMID: 25821033

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chains: A, B
Molecule details ›
Chains: A, B
Length: 56 amino acids
Theoretical weight: 6.3 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 304-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 74.287Å b: 74.287Å c: 41.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.201 0.256
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Site-directed spin labeling of proteins for distance measurements in vitro and in cells.
Roser et al. (2016)
9 more

6 mentions without citation

The double-histidine Cu²⁺-binding motif: a highly rigid, site-specific spin probe for electron spin resonance distance measurements.
Cunningham et al. (2015)
5 more