› 4wdd

X-ray diffraction
2.1Å resolution

Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation T232A, complexed with citrate

Released:
DOI: 10.2210/pdb4wdd/pdb
PDB entry 4wdd coloured by chain, front view.
PDB entry 4wdd coloured by chain, side view.
PDB entry 4wdd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Determinants of ligand binding and catalytic activity in the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase.
Raasakka A, Myllykoski M, Laulumaa S, Lehtimäki M, Härtlein M, Moulin M, Kursula I, Kursula P
Sci Rep 5 16520 (2015)
PMID: 26563764

Function and Biology Details

Reaction catalysed: 
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 2'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2',3'-cyclic-nucleotide 3'-phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16330 (Residues: 179-398; Coverage: 52%)
Gene names: Cnp, Cnp1
Sequence domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP or CNPase)
Structure domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase superfamily

Ligands and Environments

3 bound ligands:
Ligand CIT 1 x CIT
Ligand CL 2 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 42.23Å b: 46.03Å c: 106.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Flexible Players within the Sheaths: The Intrinsically Disordered Proteins of Myelin in Health and Disease.
Raasakka et al. (2020)
2 more

1 mention without citation

Determinants of ligand binding and catalytic activity in the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase.
Raasakka et al. (2015)