› 4wan

X-ray diffraction
1.8Å resolution

Crystal structure of Msl5 protein in complex with RNA at 1.8 A

Released:
DOI: 10.2210/pdb4wan/pdb
PDB entry 4wan coloured by chain, front view.
PDB entry 4wan coloured by chain, side view.
PDB entry 4wan coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.
Jacewicz A, Chico L, Smith P, Schwer B, Shuman S
RNA 21 401-14 (2015)
PMID: 25587180

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171313 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Branchpoint-bridging protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 129 amino acids
Theoretical weight: 14.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12186 (Residues: 144-271; Coverage: 27%)
Gene names: BBP, L2949, MSL5, SF1, YLR116W
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
RNA (5'-R(P*UP*AP*CP*UP*AP*AP*CP*A)-3') Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 5 x GOL
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 53.38Å b: 130.97Å c: 53.37Å
α: 90° β: 117.43° γ: 90°
R-values:
R R work R free
0.18 0.175 0.206
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.
DeHaven et al. (2016)
1 more

1 mention without citation

Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.
Jacewicz et al. (2015)