› 4w8w

X-ray diffraction
2.8Å resolution

Crystal structure of oligomeric Cmr4 from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb4w8w/pdb
PDB entry 4w8w coloured by chain, front view.
PDB entry 4w8w coloured by chain, side view.
PDB entry 4w8w coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural model of a CRISPR RNA-silencing complex reveals the RNA-target cleavage activity in Cmr4.
Benda C, Ebert J, Scheltema RA, Schiller HB, Baumgärtner M, Bonneau F, Mann M, Conti E
Mol Cell 56 43-54 (2014)
PMID: 25280103

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system Cmr endoribonuclease Cmr4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 312 amino acids
Theoretical weight: 34.71 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8U1S9 (Residues: 1-295; Coverage: 100%)
Gene names: PF1126, cmr4
Sequence domains: RAMP superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 63.707Å b: 129.509Å c: 195.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

28 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
27 more