› 4v2m

X-ray diffraction
1.84Å resolution

Crystallographic structure of thioredoxin from Litopenaeus vannamei: Radiation damage effect at 34 MGy, focused in disulfide bonds.

Released:
DOI: 10.2210/pdb4v2m/pdb
PDB entry 4v2m coloured by chain, front view.
PDB entry 4v2m coloured by chain, side view.
PDB entry 4v2m coloured by chain, top view.
Source organism: Penaeus vannamei
Primary publication:
Crystallographic studies evidencing the high energy tolerance to disrupting the interface disulfide bond of thioredoxin 1 from white leg shrimp Litopenaeus vannamei.
Campos-Acevedo AA, Rudiño-Piñera E
Molecules 19 21113-26 (2014)
PMID: 25517346

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 11.96 KDa
Source organism: Penaeus vannamei
Expression system: Escherichia coli
UniProt:
  • Canonical: B1PWB9 (Residues: 1-105; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3212
Unit cell:
a: 57.216Å b: 57.216Å c: 117.952Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.217
Expression system: Escherichia coli

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Citations

1 review citation

Protein Promiscuity in H2O2 Signaling.
Young et al. (2019)
1 other article