› 4ufs

X-ray diffraction
4.8Å resolution

Low resolution structure R-spondin-2 (Fu1Fu2) in complex with the ectodomains of LGR5 and ZNRF3

Released:
DOI: 10.2210/pdb4ufs/pdb
PDB entry 4ufs coloured by chain, front view.
PDB entry 4ufs coloured by chain, side view.
PDB entry 4ufs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of R-spondin 2 in complex with the ectodomains of its receptors LGR5 and ZNRF3.
Zebisch M, Jones EY
J Struct Biol 191 149-55 (2015)
PMID: 26123262

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130977 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Leucine-rich repeat-containing G-protein coupled receptor 5 Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 54.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75473 (Residues: 32-486, 563-565, 566-566; Coverage: 52%)
Gene names: GPR49, GPR67, LGR5
Sequence domains:
R-spondin-2 Chain: B
Molecule details ›
Chain: B
Length: 120 amino acids
Theoretical weight: 13.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8BFU0 (Residues: 39-144; Coverage: 48%)
Gene name: Rspo2
Sequence domains: Furin-like repeat, cysteine-rich
E3 ubiquitin-protein ligase ZNRF3 Chain: C
Molecule details ›
Chain: C
Length: 165 amino acids
Theoretical weight: 18.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9ULT6 (Residues: 56-207; Coverage: 17%)
Gene names: KIAA1133, RNF203, ZNRF3
Sequence domains: ZNRF-3 Ectodomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I422
Unit cell:
a: 188.609Å b: 188.609Å c: 165.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.27 0.313
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Structural Perspectives on Axon Guidance.
Seiradake et al. (2016)
3 more

3 mentions without citation

Crystal structure of R-spondin 2 in complex with the ectodomains of its receptors LGR5 and ZNRF3.
Zebisch et al. (2015)
2 more