› 4uea

X-ray diffraction
2.62Å resolution

Complex of D. melanogaster eIF4E with a designed 4E-binding protein (Form I)

Released:
DOI: 10.2210/pdb4uea/pdb
PDB entry 4uea coloured by chain, front view.
PDB entry 4uea coloured by chain, side view.
PDB entry 4uea coloured by chain, top view.
Source organisms:
Primary publication:
Molecular architecture of 4E-BP translational inhibitors bound to eIF4E.
Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, Weichenrieder O, Izaurralde E
Mol Cell 57 1074-1087 (2015)
PMID: 25702871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155765 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 184 amino acids
Theoretical weight: 21.25 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48598 (Residues: 80-259; Coverage: 70%)
Gene names: CG4035, Eif4e, eIF-4E, eIF4E1
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
DESIGNED 4E-BP Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 44 amino acids
Theoretical weight: 5.1 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P61
Unit cell:
a: 94.54Å b: 94.54Å c: 131.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.205 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Modulation of Intrinsically Disordered Protein Function by Post-translational Modifications.
Bah et al. (2016)
16 more