› 4ucv

X-ray diffraction
2.6Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.51 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43813 (Residues: 1-324; Coverage: 48%)
Gene names: HI_1100, lig, ligA, ligN
Sequence domains: NAD-dependent DNA ligase adenylation domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IWH 1 x IWH
Ligand I6G 1 x I6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 139.16Å b: 139.16Å c: 56.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.257 0.28
Expression system: Escherichia coli

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