› 4u3v

X-ray diffraction
1.73Å resolution

Crystal structure of the trans-acyltransferase polyketide synthase enoyl-isomerase

Released:
DOI: 10.2210/pdb4u3v/pdb
PDB entry 4u3v coloured by chain, front view.
PDB entry 4u3v coloured by chain, side view.
PDB entry 4u3v coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
A double-hotdog with a new trick: structure and mechanism of the trans-acyltransferase polyketide synthase enoyl-isomerase.
Gay DC, Spear PJ, Keatinge-Clay AT
ACS Chem Biol 9 2374-81 (2014)
PMID: 25089587

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase PksR Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.66 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O31784 (Residues: 1124-1395; Coverage: 11%)
Gene names: BSU17220, pksR
Sequence domains:
Structure domains: Polyketide synthase dehydratase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 48.821Å b: 68.267Å c: 77.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Biosynthesis of polyketides by trans-AT polyketide synthases.
Helfrich et al. (2016)
7 more

3 mentions without citation

A double-hotdog with a new trick: structure and mechanism of the trans-acyltransferase polyketide synthase enoyl-isomerase.
Gay et al. (2014)
2 more