› 4u1f

X-ray diffraction
2.2Å resolution

Crystal structure of middle domain of eukaryotic translation initiation factor eIF3b

Released:
DOI: 10.2210/pdb4u1f/pdb
PDB entry 4u1f coloured by chain, front view.
PDB entry 4u1f coloured by chain, side view.
PDB entry 4u1f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CHS, Cimermančič P, Boehringer D, Sali A, Aebersold R, Ban N
Cell 158 1123-1135 (2014)
PMID: 25171412

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 3 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 56.94 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P06103 (Residues: 172-665; Coverage: 65%)
Gene names: CDC63, PRT1, YOR361C
Sequence domains: Eukaryotic translation initiation factor eIF2A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 60.16Å b: 175.87Å c: 71.32Å
α: 90° β: 113.62° γ: 90°
R-values:
R R work R free
0.19 0.187 0.226
Expression system: Escherichia coli

Quick links

Citations

39 review citations

The nuclear pore complex: understanding its function through structural insight.
Beck et al. (2017)
38 more

2 mentions without citation

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae.
Dever et al. (2016)
1 more