› 4u1c

X-ray diffraction
3.5Å resolution

Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer

Released:
DOI: 10.2210/pdb4u1c/pdb
PDB entry 4u1c coloured by chain, front view.
PDB entry 4u1c coloured by chain, side view.
PDB entry 4u1c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CHS, Cimermančič P, Boehringer D, Sali A, Aebersold R, Ban N
Cell 158 1123-1135 (2014)
PMID: 25171412

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152332 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 3 subunit C Chain: C
Molecule details ›
Chain: C
Length: 567 amino acids
Theoretical weight: 65.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32497 (Residues: 247-812; Coverage: 70%)
Gene names: NIP1, YM9924.01C, YM9952.11C, YMR309C
Sequence domains:
Eukaryotic translation initiation factor 3 subunit A Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.68 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38249 (Residues: 225-495; Coverage: 28%)
Gene names: RPG1, TIF32, YBR0734, YBR079C
Structure domains: UVR domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 155.55Å b: 155.55Å c: 91.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.26 0.293
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

The nuclear pore complex: understanding its function through structural insight.
Beck et al. (2017)
38 more

3 mentions without citation

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae.
Dever et al. (2016)
2 more