› 4u0s

X-ray diffraction
2.49Å resolution

Structure of Eukaryotic fic domain containing protein with ADP

Released:
DOI: 10.2210/pdb4u0s/pdb
PDB entry 4u0s coloured by chain, front view.
PDB entry 4u0s coloured by chain, side view.
PDB entry 4u0s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions.
Bunney TD, Cole AR, Broncel M, Esposito D, Tate EW, Katan M
Structure 22 1831-1843 (2014)
PMID: 25435325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein adenylyltransferase FICD Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 39.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BVA6 (Residues: 102-445; Coverage: 75%)
Gene names: FICD, HIP13, HYPE, UNQ3041/PRO9857
Sequence domains: Fic/DOC family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 71.04Å b: 76.01Å c: 92.03Å
α: 90° β: 107.56° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression system: Escherichia coli

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Citations

5 review citations

Early Events in the Endoplasmic Reticulum Unfolded Protein Response.
Preissler et al. (2019)
4 more

2 mentions without citation

A Novel Fic (Filamentation Induced by cAMP) Protein from Clostridium difficile Reveals an Inhibitory Motif-independent Adenylylation/AMPylation Mechanism.
Dedic et al. (2016)
1 more