› 4twd

X-ray diffraction
3.2Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with memantine

Released:
DOI: 10.2210/pdb4twd/pdb
PDB entry 4twd coloured by chain, front view.
PDB entry 4twd coloured by chain, side view.
PDB entry 4twd coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
The prokaryote ligand-gated ion channel ELIC captured in a pore blocker-bound conformation by the Alzheimer's disease drug memantine.
Ulens C, Spurny R, Thompson AJ, Alqazzaz M, Debaveye S, Han L, Price K, Villalgordo JM, Tresadern G, Lynch JW, Lummis SC
Structure 22 1399-407 (2014)
PMID: 25199693

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.28 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 377 12 x 377
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 105.482Å b: 265.284Å c: 111.098Å
α: 90° β: 110.28° γ: 90°
R-values:
R R work R free
0.202 0.199 0.252
Expression system: Escherichia coli

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Citations

1 review citation

Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.
Hanashima et al. (2021)
5 other articles

7 mentions without citation

Structure and Pharmacologic Modulation of Inhibitory Glycine Receptors.
Burgos et al. (2016)
6 more