› 4ruu

X-ray diffraction
1.4Å resolution

Crystal structure of the Q108K:K40L mutant of human Cellular Retinol Binding ProteinII in complex with All-trans-Retinal after 24 hour incubation at 1.4 Angstrom Resolution

Released:
DOI: 10.2210/pdb4ruu/pdb
PDB entry 4ruu coloured by chain, front view.
PDB entry 4ruu coloured by chain, side view.
PDB entry 4ruu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tuning the electronic absorption of protein-embedded all-trans-retinal.
Wang W, Nossoni Z, Berbasova T, Watson CT, Yapici I, Lee KS, Vasileiou C, Geiger JH, Borhan B
Science 338 1340-3 (2012)
PMID: 23224553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 29.479Å b: 36.57Å c: 64.093Å
α: 86.54° β: 86.6° γ: 64.83°
R-values:
R R work R free
0.209 0.207 0.238
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
13 more

1 mention without citation

A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
Nosrati et al. (2016)