› 4rs8

X-ray diffraction
2.29Å resolution

Apo structure of novel pNOB8 plasmid centromere binding protein

Released:
DOI: 10.2210/pdb4rs8/pdb
PDB entry 4rs8 coloured by chain, front view.
PDB entry 4rs8 coloured by chain, side view.
PDB entry 4rs8 coloured by chain, top view.
Source organism: Sulfolobus sp. NOB8H2
Primary publication:
Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castañeda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barillà D
Science 349 1120-4 (2015)
PMID: 26339031

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH arsR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 9.75 KDa
Source organism: Sulfolobus sp. NOB8H2
Expression system: Escherichia coli
UniProt:
  • Canonical: O93706 (Residues: 9-92; Coverage: 90%)
Sequence domains: Sugar-specific transcriptional regulator TrmB
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 34.3Å b: 63.6Å c: 86.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.258
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Mechanisms of gene flow in archaea.
Wagner et al. (2017)
6 more

1 mention without citation

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
Schumacher et al. (2015)