› 4ro9

X-ray diffraction
2Å resolution

2.0A resolution structure of SRPN2 (S358E) from Anopheles gambiae

Released:
DOI: 10.2210/pdb4ro9/pdb
PDB entry 4ro9 coloured by chain, front view.
PDB entry 4ro9 coloured by chain, side view.
PDB entry 4ro9 coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
Structural and inhibitory effects of hinge loop mutagenesis in serpin-2 from the malaria vector Anopheles gambiae.
Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K
J Biol Chem 290 2946-56 (2015)
PMID: 25525260

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease inhibitor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 397 amino acids
Theoretical weight: 45.41 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7QIJ8 (Residues: 22-409; Coverage: 100%)
Gene names: 1270169, AgaP_AGAP006911, SRPN2
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Unit cell:
a: 171.329Å b: 42.264Å c: 185.623Å
α: 90° β: 116.94° γ: 90°
R-values:
R R work R free
0.193 0.19 0.238
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Serpins in arthropod biology.
Meekins et al. (2017)
5 other articles

1 mention without citation

Structural and inhibitory effects of hinge loop mutagenesis in serpin-2 from the malaria vector Anopheles gambiae.
Zhang et al. (2015)