› 4rkf

X-ray diffraction
1.5Å resolution

Drosophila melanogaster Rab3 bound to GMPPNP

Released:
DOI: 10.2210/pdb4rkf/pdb
PDB entry 4rkf coloured by chain, front view.
PDB entry 4rkf coloured by chain, side view.
PDB entry 4rkf coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structures of Drosophila melanogaster Rab2 and Rab3 bound to GMPPNP.
Lardong JA, Driller JH, Depner H, Weise C, Petzoldt A, Wahl MC, Sigrist SJ, Loll B
Acta Crystallogr F Struct Biol Commun 71 34-40 (2015)
PMID: 25615965

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.76 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25228 (Residues: 1-188; Coverage: 86%)
Gene names: CG7576, Rab3
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand 1PE 4 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P212121
Unit cell:
a: 37.23Å b: 80.47Å c: 123.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Mutational Analysis of Rab3 Function for Controlling Active Zone Protein Composition at the Drosophila Neuromuscular Junction.
Chen et al. (2015)
2 more

1 mention without citation

Structures of Drosophila melanogaster Rab2 and Rab3 bound to GMPPNP.
Lardong et al. (2015)