› 4rjx

X-ray diffraction
1.54Å resolution

Crystal structure of the OprO mutant protein F62Y/D114Y

Released:
DOI: 10.2210/pdb4rjx/pdb
PDB entry 4rjx coloured by chain, front view.
PDB entry 4rjx coloured by chain, side view.
PDB entry 4rjx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure, Dynamics, and Substrate Specificity of the OprO Porin from Pseudomonas aeruginosa.
Modi N, Ganguly S, Bárcena-Uribarri I, Benz R, van den Berg B, Kleinekathöfer U
Biophys J 109 1429-38 (2015)
PMID: 26445443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin O Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 47.35 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P32977 (Residues: 25-438; Coverage: 100%)
Gene names: PA3280, oprO
Sequence domains: Phosphate-selective porin O and P
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand C8E 4 x C8E
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R3
Unit cell:
a: 87.479Å b: 87.479Å c: 159.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Understanding antibiotic resistance via outer membrane permeability.
Ghai et al. (2018)
4 more

1 mention without citation

Conversion of OprO into an OprP-like Channel by Exchanging Key Residues in the Channel Constriction.
Ganguly et al. (2017)