› 4rif

X-ray diffraction
1.85Å resolution

Landomycin Glycosyltransferase LanGT2, carbasugar substrate complex

Released:
DOI: 10.2210/pdb4rif/pdb
PDB entry 4rif coloured by chain, front view.
PDB entry 4rif coloured by chain, side view.
PDB entry 4rif coloured by chain, top view.
Source organism: Streptomyces cyanogenus
Primary publication:
Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2.
Tam HK, Härle J, Gerhardt S, Rohr J, Wang G, Thorson JS, Bigot A, Lutterbeck M, Seiche W, Breit B, Bechthold A, Einsle O
Angew Chem Int Ed Engl 54 2811-5 (2015)
PMID: 25581707

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl transferase homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 40.41 KDa
Source organism: Streptomyces cyanogenus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZGC0 (Residues: 1-373; Coverage: 100%)
Gene name: lanGT2
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand 3R2 2 x 3R2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 57.97Å b: 59.71Å c: 71.99Å
α: 87.3° β: 73.97° γ: 64.92°
R-values:
R R work R free
0.177 0.176 0.198
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
2 more