› 4rdr

X-ray diffraction
2.47Å resolution

Structure of the bacterial Zn-transporter ZnuD from Neisseria meningitidis (locked conformation bound to zinc and cadmium ions)

Released:
DOI: 10.2210/pdb4rdr/pdb
PDB entry 4rdr coloured by chain, front view.
PDB entry 4rdr coloured by chain, side view.
PDB entry 4rdr coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD.
Calmettes C, Ing C, Buckwalter CM, El Bakkouri M, Chieh-Lin Lai C, Pogoutse A, Gray-Owen SD, Pomès R, Moraes TF
Nat Commun 6 7996 (2015)
PMID: 26282243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable TonB-dependent receptor NMB0964 Chain: A
Molecule details ›
Chain: A
Length: 748 amino acids
Theoretical weight: 84.71 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JZN9 (Residues: 25-758; Coverage: 100%)
Gene name: NMB0964
Sequence domains:
Structure domains: TonB-dependent receptor, plug domain

Ligands and Environments

6 bound ligands:
Ligand CD 2 x CD
Ligand ZN 1 x ZN
Ligand C8E 5 x C8E
Ligand SO4 21 x SO4
Ligand GOL 4 x GOL
Ligand EDO 5 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I222
Unit cell:
a: 101.073Å b: 155.819Å c: 159.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
14 more

7 mentions without citation

Bacterial Strategies to Maintain Zinc Metallostasis at the Host-Pathogen Interface.
Capdevila et al. (2016)
6 more