› 4r7y

X-ray diffraction
2.7Å resolution

Crystal structure of an active MCM hexamer

Released:
DOI: 10.2210/pdb4r7y/pdb
PDB entry 4r7y coloured by chain, front view.
PDB entry 4r7y coloured by chain, side view.
PDB entry 4r7y coloured by chain, top view.
Source organisms:
Primary publication:
Analysis of the crystal structure of an active MCM hexamer.
Miller JM, Arachea BT, Epling LB, Enemark EJ
Elife 3 e03433 (2014)
PMID: 25262915

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase; Minichromosome maintenance protein MCM Chains: A, B
Molecule details ›
Chains: A, B
Length: 613 amino acids
Theoretical weight: 69.47 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UXG1 (Residues: 2-269; Coverage: 39%)
  • Canonical: Q8U3I4 (Residues: 257-966; Coverage: 33%)
Gene names: MCM, PF0482, SSO0774
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand ZN 2 x ZN
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P63
Unit cell:
a: 118.902Å b: 118.902Å c: 199.317Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.263 0.295
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Mechanisms and regulation of DNA replication initiation in eukaryotes.
Parker et al. (2017)
8 more

8 mentions without citation

The ring-shaped hexameric helicases that function at DNA replication forks.
O'Donnell et al. (2018)
7 more