› 4r6b

X-ray diffraction
2Å resolution

Rational Design of Enhanced Photoresistance in a Photoswitchable Fluorescent Protein

Released:
DOI: 10.2210/pdb4r6b/pdb
PDB entry 4r6b coloured by chain, front view.
PDB entry 4r6b coloured by chain, side view.
PDB entry 4r6b coloured by chain, top view.
Source organism: Lobophyllia hemprichii
Primary publication:
Rational design of enhanced photoresistance in a photoswitchable fluorescent protein.
Duan C, Byrdin M, El Khatib M, Henry X, Adam V, Bourgeois D
Methods Appl Fluoresc 3 014004 (2015)
PMID: 29148481

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green to red photoconvertible GFP-like protein EosFP Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 230 amino acids
Theoretical weight: 26.48 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5S6Z9 (Residues: 1-226; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 15 x SO4
Ligand SO3 4 x SO3
1 modified residue:
Ligand CR8 4 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 86.701Å b: 96.35Å c: 139.426Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.192 0.224
Expression system: Escherichia coli BL21(DE3)

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