› 4qwo

X-ray diffraction
1.52Å resolution

1.52 Angstrom Crystal Structure of A42R Profilin-like Protein from Monkeypox Virus Zaire-96-I-16

Released:
DOI: 10.2210/pdb4qwo/pdb
PDB entry 4qwo coloured by chain, front view.
PDB entry 4qwo coloured by chain, side view.
PDB entry 4qwo coloured by chain, top view.
Source organism: Monkeypox virus Zaire-96-I-16
Primary publication:
Structure of the Monkeypox virus profilin-like protein A42R reveals potential functional differences from cellular profilins.
Minasov G, Inniss NL, Shuvalova L, Anderson WF, Satchell KJF
Acta Crystallogr F Struct Biol Commun 78 371-377 (2022)
PMID: 36189721

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Profilin Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18 KDa
Source organism: Monkeypox virus Zaire-96-I-16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8V4T7 (Residues: 1-133; Coverage: 100%)
Gene names: A42R, OPG171
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

4 bound ligands:
Ligand PE8 1 x PE8
Ligand EDO 2 x EDO
Ligand CL 1 x CL
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 41.617Å b: 50.498Å c: 119.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Fragment-Based Approaches Identified Tecovirimat-Competitive Novel Drug Candidate for Targeting the F13 Protein of the Monkeypox Virus.
Ali et al. (2023)
1 more

16 mentions without citation

Emergence of monkeypox: a worldwide public health crisis.
Begum et al. (2023)
15 more