› 4qig

X-ray diffraction
3.3Å resolution

Crystal Structure of PduA with edge mutation K26A and pore mutation S40C

Released:
DOI: 10.2210/pdb4qig/pdb
PDB entry 4qig coloured by chain, front view.
PDB entry 4qig coloured by chain, side view.
PDB entry 4qig coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Selective molecular transport through the protein shell of a bacterial microcompartment organelle.
Chowdhury C, Chun S, Pang A, Sawaya MR, Sinha S, Yeates TO, Bobik TA
Proc Natl Acad Sci U S A 112 2990-5 (2015)
PMID: 25713376

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial microcompartment shell protein PduA Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 102 amino acids
Theoretical weight: 10.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A1C7 (Residues: 2-94; Coverage: 99%)
Gene names: STM2038, pduA
Sequence domains: BMC domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: F23
Unit cell:
a: 235.44Å b: 235.44Å c: 235.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.232
Expression system: Escherichia coli

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Citations

11 review citations

Enzymatic reactions in confined environments.
Küchler et al. (2016)
10 more

1 mention without citation

Selective molecular transport through the protein shell of a bacterial microcompartment organelle.
Chowdhury et al. (2015)