4qc4

X-ray diffraction
1.49Å resolution

Crystal structure of C117S mutant of human acidic fibroblast growth factor

Released:
Source organism: Homo sapiens
Primary publication:
Mutation choice to eliminate buried free cysteines in protein therapeutics.
J Pharm Sci 104 566-76 (2015)
PMID: 25312595

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-138603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05230 (Residues: 16-155; Coverage: 90%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2221
Unit cell:
a: 73.493Å b: 95.223Å c: 106.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 0.182
Expression system: Escherichia coli BL21(DE3)