› 4q2h

X-ray diffraction
1.8Å resolution

Crystal structure of probable proline racemase from agrobacterium radiobacter K84, TARGET EFI-506561, with bound carbonate

Released:
DOI: 10.2210/pdb4q2h/pdb
PDB entry 4q2h coloured by chain, front view.
PDB entry 4q2h coloured by chain, side view.
PDB entry 4q2h coloured by chain, top view.
Source organism: Agrobacterium radiobacter K84
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Stead M, Washington E, Glenn AS, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyproline 2-epimerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 39.41 KDa
Source organism: Agrobacterium radiobacter K84
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9J8G8 (Residues: 1-345; Coverage: 100%)
Gene name: Arad_0731
Sequence domains: Proline racemase
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand BCT 2 x BCT
Ligand GOL 1 x GOL
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 67.43Å b: 129.47Å c: 178.703Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.147 0.176
Expression system: Escherichia coli BL21(DE3)

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